par Dao-Thi, Minh-Hoa;Van Melderen, Laurence 
;De Genst, Erwin;Buts, Lieven;Ranquin, An;Wyns, Lode;Loris, Remy
Référence Acta crystallographica. Section D, Biological crystallography, 60, Pt 6, page (1132-1134)
Publication Publié, 2004-06
;De Genst, Erwin;Buts, Lieven;Ranquin, An;Wyns, Lode;Loris, RemyRéférence Acta crystallographica. Section D, Biological crystallography, 60, Pt 6, page (1132-1134)
Publication Publié, 2004-06
Article révisé par les pairs
| Résumé : | Plasmid addiction systems consist of a plasmid-encoded toxin-antidote pair that serves to stabilize low-copy-number plasmids in bacterial populations. CcdB, the toxin from the ccd system on the Escherichia coli F plasmid, acts as a gyrase poison. A 14 kDa fragment of gyrase, GyrA14, was found to bind to the toxin CcdB with an affinity of 1.75 x 10(-8) M. Crystals of the (GyrA14)(2) dimer in its free state belong to space group P4(3)2(1)2, with unit-cell parameters a = 86.4, c = 89.4 angstroms, and diffract to 2.4 angstroms. Crystals of the (GyrA14)(2)-(CcdB)(2) complex belong to space group P2(1)2(1)2(1), with a = 52.1, b = 83.3, c = 110.9 angstroms, and diffract to 2.8 angstroms resolution. |
