par Tonon, Sandrine 
;Badran, Bassam ;Benghiat, Fleur ;Goriely, Stanislas ;Flamand, Véronique ;Willard-Gallo, Karen ;Willems, Fabienne ;Goldman, Michel ;De Wit, Dominique
Référence European Journal of Immunology, 36, 7, page (1794-1804)
Publication Publié, 2006-07
;Badran, Bassam ;Benghiat, Fleur ;Goriely, Stanislas ;Flamand, Véronique ;Willard-Gallo, Karen ;Willems, Fabienne ;Goldman, Michel ;De Wit, Dominique Référence European Journal of Immunology, 36, 7, page (1794-1804)
Publication Publié, 2006-07
Article révisé par les pairs
| Résumé : | Pertussis toxin (PTX) is known to be mitogenic for T lymphocytes, but its direct action on naive human T cells has not been specified. Herein, we show that PTX induces the proliferation of purified adult CD45RA(+)CD4(+) T cells independently of its ADP-ribosyltransferase activity. PTX directly induces TNF-alpha and IL-2 mRNA expression, modulates the level of several cell surface receptors and induces Forkhead box p3 (Foxp3) protein accumulation in naive CD4(+) T cells. Addition of autologous dendritic cells was found to be required for the production of high levels of IFN-gamma by PTX-stimulated naive T cells. These effects of PTX occurred in conjunction with activation of NF-kappaB and NFAT transcription factors. Overall, responses of neonatal CD4(+) T cells to PTX were similar to those of adult CD45RA(+)CD4(+) naive T cells except for their blunted CD40 ligand up-regulation. We suggest that the adjuvant properties of PTX during primary cell-mediated immune responses involve a direct action on naive T lymphocytes in addition to activation of antigen-presenting cells. |
