In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter.

Dubois, Evelyne; Messenguy, Francine

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In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter.

par Dubois, Evelyne

;Messenguy, Francine

Référence Molecular and cellular biology, 11, 4, page (2162-2168)
Publication Publié, 1991-04

Article révisé par les pairs

Résumé :

ARGRI, ARGRII, and ARGRIII regulatory proteins control the expression of arginine anabolic and catabolic genes in Saccharomyces cerevisiae. We show here that they are also required in vitro to observe a protein-DNA complex with the promoter of the ARG5,6 gene. The specific binding of ARGR proteins in vitro is stimulated by arginine. Antibodies raised against a synthetic MCM1 polypeptide retard the migration of ARGR-DNA complex on gel mobility shift assays. This result suggests that MCM1 could be an additional regulatory element of arginine metabolism.

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