• Citer

Single-cell imaging of liver metabolic dynamics using fluorescent biosensors

par Perez Chavez, Israel ;Gilglioni, Eduardo Hideo ;Ezeriņa, Daria;Messens, Joris;Gurzov, Esteban Nicolas
Référence Trends in endocrinology and metabolism, 0, 0, page (0)
Publication Publié, 2025-11-01

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

Traditional metabolic studies rely on bulk tissue analyses, masking the cellular heterogeneity that underlies disease progression. Genetically encoded fluorescent biosensors now enable real-time, single-cell imaging of dynamic metabolic processes in the liver. These tools provide insights into the metabolic reprogramming in conditions such as chronic obesity, metabolic dysfunction-associated steatotic liver disease (MASLD), and hepatocellular carcinoma (HCC). By tracking specific metabolites involved in glycolysis, lipid oxidation, and the tricarboxylic acid (TCA) cycle, biosensors can reveal how these pathways respond to diverse stimuli. In this review we outline the core principles of fluorescent biosensors, provide specific recommendations for their usage, suggest possible applications in liver metabolism research, and discuss current technical challenges as well as emerging opportunities in this rapidly advancing field.