par Nielsen, Troels Tolstrup;Van Marion, Ingrid 
;Hasholt, Lis;Lundberg, Cecilia
Référence The journal of gene medicine, 11, 7, page (559-569)
Publication Publié, 2009-07
;Hasholt, Lis;Lundberg, CeciliaRéférence The journal of gene medicine, 11, 7, page (559-569)
Publication Publié, 2009-07
Article révisé par les pairs
| Résumé : | Viral vectors have been used in several different settings for the delivery of small hairpin (sh) RNAs. However, most vectors have utilized ubiquitously-expressing polymerase (pol) III promoters to drive expression of the hairpin as a result of the strict requirement for precise transcriptional initiation and termination. Recently, pol II promoters have been used to construct vectors for RNA interference (RNAi). By embedding the shRNA into a micro RNA-context (miRNA) the endogenous miRNA processing machinery is exploited to achieve the mature synthetic miRNA (smiRNA), thereby expanding the possible promoter choices and eventually allowing cell type specific down-regulation of target genes. |
