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Enzyme-immobilized and fluorinated hydrogel for enhanced oxygen retention in hypoxia treatment

par Ghaffari Bohlouli, Pejman ;Jafari, Hafez ;Shavandi, Armin
Référence Applied Materials Today, 45, page (102781)
Publication Publié, 2025-05-08

Article révisé par les pairs

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Résumé :

Hypoxia and accumulation of reactive oxygen species (ROS) impact extracellular matrix (ECM) synthesis, collagen production, and capillary angiogenesis, therefore impeding wound healing. Calcium peroxide (CPO) has been studied to alleviate hypoxia, but its rapid decomposition into oxygen (O2) and hydrogen peroxide (H2O2) limits its use. To alleviate hypoxia and ROS-related challenges, we designed a 3D hydrogel based on modified hyaluronic acid (HA). Catalase (CAT) stabilized on HA was incorporated to decompose H2O2 into O2. Additionally, fluorinated CPO-loaded polycaprolactone (PCL) particles were included for O2 generation and retention. Fluorinated particles were synthesized in two ways: by loading pentadecafluorooctanoyl chloride (PFC) into CPO-loaded PCL particles (PFC-loaded particles), or by combining CPO-loaded particles with immobilized PFC nanoparticles (PFC-conjugated particles). The hydrogels containing the fluorinated particles generated 63.9–73.4 µM of O2 and preserved 30.7–45.3 µM of the generated O2. By combining hypoxia alleviation with ROS scavenging, the hydrogels showed cell viability, and intracellular O2 level, highlighting the potential for skin wound healing.