Article révisé par les pairs
Résumé : ABSTRACT The early life period is increasingly being recognized as a window of opportunity to shape immunity, where microbiota and related probiotics have an important impact. Innate γδ T cells are the first T cells generated in utero, populating epithelial tissues such as the lung and contributing to tissue protection through, for example, IL17 production. Here, we studied the influence of maternal microbiota and probiotic supplementation during pregnancy on innate γδ T cells in the lung and thymus of newborn mice. Detailed time‐kinetic experiments showed that at birth, the murine lung T cell population was specifically dominated by IL17‐committed γδ T cells expressing an invariant Vγ6Vδ1 TCR. Single‐cell RNA‐sequencing showed that the biased IL17‐commitment of perinatal lung γδT cells is highly conserved between mice and humans. While maternal microbiota depletion with antibiotics tended to decrease the frequency of the lung Vγ6 T cells of the offspring at birth, the maternal administration of Lacticaseibacillus rhamnosus ( L.rhm .), but not of Bifidobacterium animalis subsp . lactis ( B.lac .), increased significantly their frequency, resulting in the augmentation of the IL17‐commitment of the mouse lung T cell compartment. Altogether, our data indicate that the maternal microbiota contributes to the shaping of IL17‐committed γδT cells in the lungs of newborns and that maternal administration of specific probiotic strains can enhance this process.

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