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Evaluation of sperm capacitation status from swim-up in different media using both fresh and frozen samples

par Pening, David ;de Prelle de la Nieppe, Bertrand ;Delbaere, Anne ;Demeestere, Isabelle ;Lybaert, Pascale
Référence American Society of Andrology (April 20-23, 2023: Boston)
Publication Publié, 2023-04-20

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Résumé :

Different techniques were evaluated to measure human sperm membrane potential (spectrofluorimetry and flowcytometry) from both fresh and frozen-thawed samples after swim-up in two different capacitating media. Nodifferences were detected between the technique used to measure the average membrane potential. Fresh spermsamples incubated in Sperm Medium® were more hyperpolarized compared to an incubation in Human Tubal Fluidmedia. The percentage of acrosome-reacted sperm was higher in frozen samples for all conditions.