par Yongabi, Derick;Khorshid, Mehran;Losada Perez, Patricia ;Bakhshi Sichani, Soroush;Jooken, Stijn;Stilman, Wouter;Theßeling, Florian;Martens, Tobie;Van Thillo, Toon;Verstrepen, Kevin ;Dedecker, Peter;Vanden Berghe, Pieter;Lettinga, Minne Paul;Bartic, Carmen;Lieberzeit, Peter;Schöning, Michael;Thoelen, Ronald;Fransen, Marc;Wübbenhorst, Michael;Wagner, Patrick
Référence Advanced Science, 9, page (2200459)
Publication Publié, 2022-07-03
Référence Advanced Science, 9, page (2200459)
Publication Publié, 2022-07-03
Article révisé par les pairs
Résumé : | Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label-free methods without (bio)-chemical markers or surface-engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label- and receptor-free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell-detachment with sharply defined time-patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell-type-specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic-type oscillations are observed during detachment of mammalian and yeast-cell ensembles, providing additional cell-specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs. |