par Suarez Gonzalez, Nathalie ;Sonet, Gontran ;Eeckhout, Peter
Référence 10th Meeting of the International Society for Biomolecular Archaeology (ISBA) (13-16/09/2032: Tartu, Estonia)
Publication Non publié, 2023-09-13
Poster de conférence
Résumé : Ancient DNA (aDNA) analysis can be a useful tool for sex determination, general mitochondrial lineage (haplogroup) and disease diagnosis in human remains. However, non-endogenous DNA contamination of archaeological material is a recurrent problematic, since excavation, handling and storage usually don't fit with the precautions recommended for ancient DNA analysis. Here, we present preliminary aDNA results from several human individuals recovered by the Ychsma Project (ULB) from the monumental archaeological site of Pachacamac, Peru, a corpus dating from 500 to 1400 AD where a high proportion of diseases is suspected. Our objectives are to complete the demographic information and to characterize the health status of the population buried in this pilgrimage site dedicated to the eponymous healing god. Our preliminary results show that, despite an important modern human-DNA contamination, a strict selection of DNA reads based on short sizes and deamination damage patterns that are typical for ancient DNA, allow us to determine the genetic sex of 70% of the individuals. The challenging detection of ancient DNA form human pathogens requires additional precautions. By multiplying negatives and environmental controls and data analysis strategies, our preliminary results suggest that our approach is appropriate for the detection of paleopathological conditions in this major archaeological corpus.