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DNA-Origami Line-Actants Control Domain Organization and Fission in Synthetic Membranes.

par Rubio-Sánchez, Roger;Mognetti, Bortolo Matteo ;Cicuta, Pietro;Di Michele, Lorenzo
Référence Journal of the American Chemical Society, 145, 20, page (11265-11275)
Publication Publié, 2023-05-01

Article révisé par les pairs

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Résumé :

Cells can precisely program the shape and lateral organization of their membranes using protein machinery. Aiming to replicate a comparable degree of control, here we introduce DNA-origami line-actants (DOLAs) as synthetic analogues of membrane-sculpting proteins. DOLAs are designed to selectively accumulate at the line-interface between coexisting domains in phase-separated lipid membranes, modulating the tendency of the domains to coalesce. With experiments and coarse-grained simulations, we demonstrate that DOLAs can reversibly stabilize two-dimensional analogues of Pickering emulsions on synthetic giant liposomes, enabling dynamic programming of membrane lateral organization. The control afforded over membrane structure by DOLAs extends to three-dimensional morphology, as exemplified by a proof-of-concept synthetic pathway leading to vesicle fission. With DOLAs we lay the foundations for mimicking, in synthetic systems, some of the critical membrane-hosted functionalities of biological cells, including signaling, trafficking, sensing, and division.