par Valiente-Gabioud, Ariel;Garteizgogeascoa Suñer, Ines 
;Idziak, Agata;Fabritius, Arne;Angibaud, Julie;Basquin, Jérome;Valentin Nägerl, U.;Singh, Sumeet Pal ;Griesbeck, Oliver
Référence BioRxiv
Publication Publié, 2023-06-01
;Idziak, Agata;Fabritius, Arne;Angibaud, Julie;Basquin, Jérome;Valentin Nägerl, U.;Singh, Sumeet Pal ;Griesbeck, OliverRéférence BioRxiv
Publication Publié, 2023-06-01
Article révisé par les pairs
| Résumé : | Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the lack of methods that allow monitoring in tight inter-cell spaces at high spatio-temporal resolution. We engineered high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (KD) ranging from 0.8 mM to 2.9 mM, making them uniquely tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo, where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms.Competing Interest StatementThe authors have declared no competing interest. |
