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Self-Generating and Preserving Oxygen and Hydrogen Peroxide Printable Hyaluronic Acid-Based reactive Oxygen Species Scavenger Hydrogel for Wound Healing

par Ghaffari Bohlouli, Pejman ;Shavandi, Amin
Référence INNOGLY & GLYCONanoPROBES(20th-21st April 2023: Iasi - Romania), International Meeting of Young Researchers – INNOGLY & GLYCONanoPROBES
Publication Publié, 2023-04-21

Abstract de conférence

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Résumé :

Chronic wounds pose a lifetime risk and require multifunctional approaches to alleviate hypoxia, compensate for insufficient nitric oxide (NO) synthesis, induce oxidative stress, scavenge reactive oxygen species (ROS), regulate the inflammatory microenvironment including biofilm formation, and deliver bioactive agents to the site of the wound. In this study, we designed a multifunctional printable hyaluronic acid (HA) hydrogel functionalized with a yeast-derived peptide that contains self-generating and preserving oxygen (O2) and hydrogen peroxide (H2O2) particles. We conjugated the yeast-derived ACE-inhibitory peptide, VW-9, onto aminated and phenolated HA to provide a continuous peptide supply at the wound site. The modification of HA was confirmed using 1H NMR and FTIR. The swelling capacity of the conjugated hydrogels was 1.5 times higher compared to the aminated HA hydrogel. The conjugated peptide did not affect the rheological properties and morphology of the hydrogels [1]. Furthermore, we introduced self-generating and preserving O2 and H2O2 fluorinated chitosan (PFC-chitosan) containing CaO2-loaded polycaprolactone (PCL) particles into the modified printable HA hydrogel. The CaO2 in PCL particles prevents decomposition, and PFC groups dissolve the generated O2, prolonging its supply time and preserving it for up to 268 hours in the release media. The particles with PFC-chitosan show higher cell viability and slower O2 release rates, indicating an improved potential for angiogenesis.The simultaneous generation of O2 and H2O2 from PFC-chitosan and delivery of the VW-9 peptide are believed to have the potential to improve chronic wound healing. Our results indicate that peptide-enriched hydrogels and PFC-chitosan particles have promising applications in wound healing.