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Inhibition or deletion of Adenosine A2A receptor enhances acetylcholine-induced vascular response: role of angiotensin-II in A2A AR-/- vs. C57Bl/6 mice

par Agba, Stephanie S.O.;Hanif, Ahmad;Ledent, Catherine ;Stephen, Tilley T.L.;Nayeem, Mohammed M.A.
Référence FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36
Publication Publié, 2022-05-01

Article révisé par les pairs

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Résumé :

In previous studies, we showed that adenosine-induced vascular relaxation was reduced in adenosine A2A receptor (A2A AR)-null (A2A AR-/- ) or A2A AR-inhibited C57Bl/6 mice. However, it is unknown the acetylcholine-induced vascular response in A2A AR-/- or A2A AR-inhibited C57Bl/6 mice; therefore, we hypothesized that the acetylcholine enhances endothelial-dependent vascular relaxation in A2A AR-gene deleted (A2A AR-/- ) or inhibited C57Bl/6 mice compared to their respective controls. Acetylcholine-induced dose dependent vascular response was tested with SCH58261 (A2A AR-antagonist) in C57Bl/6 vs. non-treated C57Bl/6 mice and angiotensin-II (Ang-II) in C57Bl/6 vs. non-treated C57Bl/6 mice, Ang-II treated A2A AR-/- vs. non-treated A2A AR-/- mice and Ang-II treated A2A AR-/- vs. Ang-II treated C57Bl/6 mice. In C57Bl/6 mice, SCH58261 (1µM) increased in acetylcholine-induced dose-dependent vascular relaxation compared to non-treated C57Bl/6 mice. Similarly, in A2A AR-/- mice, acetylcholine enhanced dose-dependent vascular relaxation compared to C57Bl/6 mice. However, acetylcholine-induced dose-dependent vascular relaxation was reduced with angiotensin-II (Ang-II,1µM) in C57Bl/6 compared to non-treated C57Bl/6 mice and acetylcholine-induced dose-dependent vascular relaxation was reduced with Ang-II (1µM) in C57Bl/6 compared to A2A AR-/- treated mice. Our data suggest that the acetylcholine dose-dependent vascular relaxation is endothelial dependent and is enhanced in the absence or inhibition of A2A AR unlike adenosine dose-dependent vascular relaxation in mice.