par Boivin, Gael;Faget, Julien;Ancey, Pierre-Benoit;Gkasti, Aspasia;Mussard, Julie;Engblom, Camilla;Pfirschke, Christina;Contat, Caroline;Pascual, Justine;Vazquez, Jessica;Bendriss-Vermare, Nathalie;Caux, Christophe;Vozenin, Marie-Catherine;Pittet, Mikael;Gunzer, Matthias;Meylan, Etienne
Référence Nature communications, 11, 1
Publication Publié, 2020-12-01
Référence Nature communications, 11, 1
Publication Publié, 2020-12-01
Article révisé par les pairs
Résumé : | Abstract Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models. |