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A fast method for in vitro biomineralization of PVA/alginate/biphasic calcium phosphate hydrogel

par Nie, Lei;Li, Xingchen;Chang, Pengbo;Liu, Shuang;Wei, Qianqian;Guo, Qingping;Wu, Qiaoyun;Fan, Lihong;Okoro, Oseweuba ;Shavandi, Amin
Référence Materials letters, 131182
Publication Publié, 2021-12-01

Article révisé par les pairs

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Résumé :

The development of biomineralized hydrogels with excellent cytocompatibility is of great importance for tissue engineering applications. Here, porous polyvinyl alcohol/alginate/biphasic calcium phosphate (BPS) hydrogels were fabricated via chemical and physical crosslinking methods, and the BPS hydrogels were in vitro biomineralized using urease in saturated calcium-phosphorus solution. For comparison, the BPS hydrogels were also treated using simulated body fluid (SBF) and Dulbecco's modified Eagle's medium with fetal bovine serum (DMEM-FBS), respectively. The physicochemical characterizations confirmed that bone-like apatite was quickly formed on the urease-biomineralized BPS hydrogels compared to the SBF and DMEM-FBS treated hydrogels. Additionally, the bone marrow derived mesenchymal stem cells (BMSCs), adhered and proliferated on the biomineralized hydrogels, were systematically analyzed using cell counting kit-8 (CCK-8). This paper demonstrated the potential urease for fast in vitro biomineralization on hydrogels to improve the cytocompatibility for bone tissue engineering applications.