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Characterization of adaptive-like γδ t cells in ugandan infants during primary cytomegalovirus infection

par Tuengel, Jessica;Ranchal, Sanya;Maslova, Alexandra;Aulakh, Gurpreet;Papadopoulou, Maria ;Drissler, Sibyl;Cai, Bing;Mohsenzadeh-Green, Cetare;Soudeyns, Hugo;Mostafavi, Sara;Elzen, Peter van den;Vermijlen, David ;Cook, Laura;Gantt, Soren
Référence Viruses, 13, 10, 1987
Publication Publié, 2021-10

Article révisé par les pairs

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Résumé :

Gamma-delta (γδ) T cells are unconventional T cells that help control cytomegalovirus (CMV) infection in adults. γδ T cells develop early in gestation, and a fetal public γδ T cell receptor (TCR) clonotype is detected in congenital CMV infections. However, age-dependent γδ T cell responses to primary CMV infection are not well-understood. Flow cytometry and TCR sequencing was used to comprehensively characterize γδ T cell responses to CMV infection in a cohort of 32 infants followed prospectively from birth. Peripheral blood γδ T cell frequencies increased during infancy, and were higher among CMV-infected infants relative to uninfected. Clustering analyses revealed associations between CMV infection and activation marker expression on adaptive-like Vδ1 and Vδ3, but not innate-like Vγ9Vδ2 γδ T cell subsets. Frequencies of NKG2C+CD57+ γδ T cells were temporally associated with the quantity of CMV shed in saliva by infants with primary infection. The public γδ TCR clonotype was only detected in CMV-infected infants <120 days old and at lower frequencies than previously described in fetal infections. Our findings support the notion that CMV infection drives age-dependent expansions of specific γδ T cell populations, and provide insight for novel strategies to prevent CMV transmission and disease.