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Injectable anti-infectious wound dressing hydrogel using marine poly, and oligo-saccharide

par Jafari, Hafez ;Delporte, Christine ;Bernaerts, Katrien;Shavandi, Amin
Référence Nature conferences - Bio-Inspired Nanomaterials (November 15-17, 2021: Seoul National University, Seoul, Korea)
Publication Non publié, 2021

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Résumé :

Given advances in the treatment of various diseases such as cancer, where specific treatment for targeted tissues or polytherapy is common, our toolkit for wound healing is relatively empty. There is an immense need for innovative biomaterials to address the acute inflammation and antibiotic-resistant organisms in infected wounds. We developed anti-infectious, injectable wound dressing hydrogels with antibacterial and antioxidant activity derived from chitooligosaccharides (COS) as bioactive compounds (developed in our lab) for chronic skin wounds. A co-enzymatically mediated crosslinking using horseradish (HRP) and glucose oxidase (GOx) triggered by glucose lead to a gradual release of hydrogen peroxide (H2O2) required for the activation of HRP, resulted in a mild enzymatic crosslinking of the phenol containing polysaccharides (chitosan, and alginate). GOx consumes the glucose and gradually releases H2O2 resulting in the activation of HRP and initializing the crosslinking; besides, glucono-delta-lactone (GDL), as a byproduct of the glucose oxidation reaction, is hydrolyzed and gradually donates protons, leading to the protonation of more free amino groups of chitosan and COS, which results in the enhancement of the electrostatic interactions with negatively charged alginate. The physiochemical investigation of the hydrogel revealed a suitable swelling ratio (<50%) and complete biodegradation of hydrogels after nine days, and a heterogenous microstructure with pore size ranging from 50 to 350 µm ideal for providing adequate space required for cell growth and nutrition transition. The hydrogel could exhibit flexibility, injectability, moldability, and self-healing property due to the dynamic electrostatic interaction as secondary crosslinking acts as a sacrificial bond for energy dissipation. Biological activities assessment showed that COS incorporation significantly improved the antioxidant (34.7 ± 2.8 to 70.5 ± 3.3), and could inhibit the growth of E. coli and S. aureus according to the colony counting assay. Besides, the hydrogel showed a promising 3D cell encapsulation of 3T3L fibroblast with uniform cell spreading and viability. Hence, the hydrogel exhibited great potential as a wound dressing hydrogel to prevent wound infections and speed up skin regeneration.