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Transcriptional profile of cytokines, regulatory mediators and tlr in mesenchymal stromal cells after inflammatory signaling and cell-passaging

par Merimi, Makram ;Buyl, Karolien;Daassi, Dhouha;Rodrigues, Robim Marcelino;Melki, Rahma;Lewalle, Philippe ;Vanhaecke, Tamara;Fahmi, Hassan;Rogiers, Vera;Lagneaux, Laurence ;De Kock, Joery;Najar, Mehdi
Référence International journal of molecular sciences, 22, 14, 7309
Publication Publié, 2021-07

Article révisé par les pairs

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Résumé :

Adult human subcutaneous adipose tissue (AT) harbors a rich population of mesenchymal stromal cells (MSCs) that are of interest for tissue repair. For this purpose, it is of utmost importance to determine the response of AT-MSCs to proliferative and inflammatory signals within the damaged tissue. We have characterized the transcriptional profile of cytokines, regulatory mediators and Toll-like receptors (TLR) relevant to the response of MSCs. AT-MSCs constitutively present a distinct profile for each gene and differentially responded to inflammation and cell-passaging. Inflammation leads to an upregulation of IL-6, IL-8, IL-1β, TNFα and CCL5 cytokine expression. Inflammation and cell-passaging increased the expression of HGF, IDO1, PTGS1, PTGS2 and TGFβ. The expression of the TLR pattern was differentially modulated with TLR 1, 2, 3, 4, 9 and 10 being increased, whereas TLR 5 and 6 downregulated. Functional enrichment analysis demonstrated a complex interplay between cytokines, TLR and regulatory mediators central for tissue repair. This profiling highlights that following a combination of inflammatory and proliferative signals, the sensitivity and responsive capacity of AT-MSCs may be significantly modified. Understanding these transcriptional changes may help the development of novel therapeutic approaches.