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Effect of oxidized low-density lipoprotein on head and neck squamous cell carcinomas

par Kindt, Nadège;Journé, Fabrice ;Carlier, Stéphane ;Trelcat, Anne;Scalia, Alessandro;Saussez, Sven
Référence Biomedicines, 9, 5, 513
Publication Publié, 2021-05

Article révisé par les pairs

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Résumé :

Cardiovascular disease (CVD) and cancer are two major causes of death worldwide. The question is, “Could there be a link between these two pathologies in addition to their shared, common risk factors?” To find some answers, we studied the effect of oxidized low-density lipo-proteins (oxLDL) on head and neck cancer (HNC) cell lines, since oxLDL is a major contributor to atherosclerosis and the principal cause of CVD. In this study, we exposed three HNC cell lines (Detroit 562, UPCI-SCC-131 and FaDu) to oxLDL. We investigated two oxLDL receptors, CD36 and Lox-1, using immunofluorescence. Cancer cell migration was evaluated using Boyden chambers and the Wnt/β-catenin pathway was investigated using Western blotting. We demonstrated that the expression of CD36 and Lox-1 significantly increases after exposure to oxLDL. Moreover, we found that oxLDL reduces the migration of HNC cell lines, an observation that is in line with an increased degradation of β-catenin under oxLDL. Finally, the inhibition of CD36 with sulfosuc-cinimidyl oleate (SSO) reverses the inhibition of cell migration. In conclusion, we report that ox-LDL seems to induce an increase in CD36 expression on HNC cell lines, enhancing the uptake of these lipids in cells to finally decrease cancer cell migration via the CD36/β-catenin pathway.