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Injectable cell-laden poly(N-isopropylacrylamide)/chitosan hydrogel reinforced via graphene oxide and incorporated with dual-growth factors

par Nie, Lei;Chen, Dong;Zhong, Shengping;Shi, Qiming;Sun, Yi;Politis, Constantinus;Shavandi, Amin
Référence Materials letters, 280, 128572
Publication Publié, 2020-12-01

Article révisé par les pairs

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Résumé :

Injectable hydrogels have gained lots of attention as cell and growth factor carrier in tissue engineering. Here, we developed a novel graphene oxide (GO) reinforced poly(N-isoproplylacrylamide)/chitosan temperature-responsive (pNCG) hydrogel, and the gelation temperature was around 36.4 °C. Then, vascular endothelial cells (VECs) laden pNCG hydrogel was fabricated by encapsulating VECs through the sol–gel transfer process. Also, a sequential release system of monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) was delicately designed and incorporated into VECs-laden pNCG hydrogel. In vitro and in vivo experiments confirmed that the VECs could proliferate into hydrogel, as well as feasible in-growing angiogenesis. Thus, the injectable pNCG hydrogel holds great potential for biomedical applications.