par Lherisson, C.;Svoboda, Michal 
;Pradayrol, Lucien;Estival, Agnes;Christophe, Jean ;Vaysse, Nicole
Référence Pancreas, 3, 6, page (668-674)
Publication Publié, 1988
;Pradayrol, Lucien;Estival, Agnes;Christophe, Jean ;Vaysse, NicoleRéférence Pancreas, 3, 6, page (668-674)
Publication Publié, 1988
Article révisé par les pairs
| Résumé : | The immunoblotting technique is applied to the analysis of "somatostatin" compounds secreted by R.I.N. T3 cells. We can confirm that two proforms of 15,300 ± 750 and 29,000 ± 1,100 Da accumulate in the extracellular medium. The unexpected form of 29 kDa, probably a dimeric form, disappears in reducing conditions. However, the 15 kDa peptide is characterized by several antibodies directed against either the intramolecular cycle of somatostatin-14 or the N-terminal extension of somatostatin-28. The 15 kDa form presents the same electrophoretic mobility in SDS-PAGE than the prosomatostatin isolated from a hypothalamic extract. Furthermore, this compound corresponds to the calculated mass of 10,388 Da deduced from the cDNA sequence. The detection of an immunoreactive 6 kDa peptide in the gel filtration fractions suggests an intermediate step in the prosomatostatin processing in these cells. © 1988 Raven Press, New York. |
