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Presence of fowl parvovirus in fibroblast cell cultures prepared from uninoculated white leghorn chicken embryos

par Kisari, János ;Rommelaere, Jean
Référence Avian pathology, 16, 1, page (115-121)
Publication Publié, 1987-01

Article révisé par les pairs

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Résumé :

Chicken embryo fibroblast cell cultures prepared from commercial uninoculated White Leghorn eggs showed a spontaneous degeneration. Upon staining with haematoxylin-eosin intranuclear inclusion bodies of Cowdry type A were seen. Also, nuclear fluorescence was detected after indirect immunofluorescence staining with anti-serum to fowl parvovirus strain ABU. Electron microscopy of thin sections revealed parvovirus-like particles in both the nucleus and cytoplasm of the affected cells. During purification the viral particles banded in CsCl at a density of 1.42 to 1.43 g/ml and displayed typical parvovirus morphology by negative-staining EM. The viral particles contained single-stranded DNA of one polarity with a natural primer at the 3'-end of the molecules. All these properties are characteristic of self-replicating fowl parvovirus strongly suggesting its transovarial transmission. © 1987, Taylor & Francis Group, LLC. All rights reserved.