par Lejong, Marie 
;Vanmuylder, Nathalie ;Choa-Duterre, M;Louryan, Stéphane
Référence Morphologie
Publication Publié, 2019-01-01
;Vanmuylder, Nathalie ;Choa-Duterre, M;Louryan, Stéphane Référence Morphologie
Publication Publié, 2019-01-01
Article révisé par les pairs
| Résumé : | Introduction: Primordial Germ Cells (PGCs) are present in all sexually reproducing animals. They differentiate into spermatozoa or oocytes and are therefore responsible for the transmission of genetic and epigenetic information across generations. In birds, PGCs are first observed in the center of the blastodisc at stage Eyal-Giladi X. With the formation of the primitive streak, germ cells are translocated anteriorly to the germinal crescent. At stage Hamburger- Hamilton 10–12, they enter the vasculature before migrating through the dorsal mesentery towards the genital ridges. Material and methods: Embryos from stages Hamburger-Hamilton (HH) 16 to 22 were collected. Blood samples were taken from the dorsal aorta and from the heart in order to perform blood smears and PAS staining. Embryos were dissected and fixed in Serra's medium. Sections were placed on slides for PAS staining. A sample of each embryo was collected for DNA extraction and PCR in order to determine the sex of the embryos. Results: PGCs were observed in blood circulation until stage HH 20 on blood smears and until stage HH 19 on histological sections. The first PGCs arrived in the genital ridges were observed from stage HH 17. A few germ cells were still migrating in the dorsal mesentery at stage HH 22. The aim of this study was to review the chronology of the migration of PGCs in chick embryos. |
