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Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey

par Dodemont, Magali ;Argudín, María Ángeles M.A.;Willekens, Julie;Vanderhelst, Eef;Pierard, Denis;Miendje Deyi, Yvette Véro ;Hanssens, Laurence ;Franckx, Hilde;Schelstraete, Petra;Leroux-Roels, Isabel;Nonhoff, Claire ;Deplano, Ariane ;Knoop, Christiane ;Malfroot, Anne;Denis, Olivier
Référence Journal of Cystic Fibrosis, 18, 1, page (86-93)
Publication Publié, 2019-01-01

Article révisé par les pairs

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Résumé :

Background: This study aims to determine the prevalence and characteristics of Staphylococcus aureus in Belgian cystic fibrosis (CF) patients. Methods: Non-duplicate respiratory samples from 510 CF-patients (2012−2013) were examined. One isolate per patient was analysed unless different phenotypes were recovered. Isolates were investigated for mecA/mecC, toxins presence, spa-typing, MLST and SCCmec-typing. Potential livestock-associated (LA) isolates were examined for their immune-evasion-cluster (IEC) genes. Results: S. aureus (n = 380), including 41 small-colony variants (SCVs), were isolated from 66.7% patients. The prevalence of methicillin-resistant S. aureus (MRSA) colonization was 4.9%. Two MRSA isolates carried toxic shock syndrome toxin 1 (TSST-1). Most MRSA (65%) belonged to two nosocomial epidemic clones (CC5, CC8) widespread in Belgium. Methicillin susceptible S. aureus (MSSA) showed great genetic diversity. Five of 33 isolates belonging to potential LA-lineages were IEC negative, including three methicillin-resistant isolates, suggesting an animal origin. Conclusions: The MRSA-prevalence in Belgian CF-patients remained constant (2001−2013), but SCV-prevalence increased. Most MRSA belonged to health-care-associated clones. Three patients carrying LA-MRSA were found, requiring further investigation to determine the risk factors for LA-MRSA acquisition.