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Surface Modification of Germanium with Oligo(ethylene glycol) Functionalized Calix[4]arene-tetradiazonium Salts Prevents Nonspecific Adsorption of Proteins

par Blond, Pascale ;Mattiuzzi, Alice ;Valkenier, Hennie ;Troian Gautier, Ludovic ;Bergamini, Jean-François;Doneux, Thomas ;Goormaghtigh, Erik ;Raussens, Vincent ;Jabin, Ivan
Référence Rencontres en Chimie Organique Biologique (17: 18 - 22 mars 2018: Aussois)
Publication Publié, 2018-03

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Résumé :

Proteins are involved in a very large number of biological processes. Biosensors that enable fast and selective identification and structural characterization of proteins in complex mixtures represent an important target for research. A major challenge in the development of such biosensors is the modification of surfaces by a robust organic monolayer able to specifically interact with a protein and displaying anti-fouling properties to prevent nonspecific adsorption phenomena. In this context, there is an increasing interest in the use of germanium-based surfaces because this material can be readily used for FTIR spectroscopy. Contrary to the other reliable detection methods, FTIR spectroscopy allows to simultaneously detect proteins and collect a wealth of information such as secondary structure and post-traductional modifications (e.g. phosphorylation, glycosylation level, etc.). Oligo(ethylene glycol) (oEG) films are well known to be efficient at reducing the nonspecific adsorption of biomolecules. However, self-assembled monolayers of thiolated oEG present important limitations related to their lack of thermal and long-term stability, narrow electrochemical window and stochastic behaviour.To prevent the nonspecific adsorption of the many proteins and biomolecules present in biological fluids, we use a strategy that consists in the modification of surfaces through the grafting of calix[4]arene-tetra-diazonium salts.1 More precisely, we have investigated the grafting on Ge and Au surfaces of monolayers of calix[4]arenes bearing oEGs substituents of different lengths. The anti-fouling properties of these modified surfaces were evaluated by FTIR spectroscopy and confocal laser scanning fluorescence microscopy upon exposure to bovine serum albumin (BSA) in phosphate (PBS) buffer.