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Grafting of Oligo(ethylene glycol) Functionalized Calix[4]arene-tetra-diazonium salts for antifouling Germanium and Gold surfaces

par Blond, Pascale ;Mattiuzzi, Alice ;Valkenier, Hennie ;Troian Gautier, Ludovic ;Bergamini, Jean-François;Doneux, Thomas ;Goormaghtigh, Erik ;Raussens, Vincent ;Jabin, Ivan
Référence International Symposium on Macrocyclic and Supramolecular Chemistry (13: 8-13 Juillet 2018: Québec)
Publication Publié, 2018-07

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Résumé :

Biosensors that enable fast and selective identification and structural characterization of proteins in complex mixtures represent an important target for research. A major challenge in the development of such biosensors is the modification of surfaces by a robust organic monolayer able to specifically interact with a protein and displaying anti-fouling properties to prevent nonspecific adsorption phenomena. In this context, there is an increasing interest in the use of germanium-based surfaces because this material can be readily used for FTIR spectroscopy. Contrary to the other reliable detection methods, FTIR spectroscopy allows to simultaneously detect proteins and collect a wealth of information such as secondary structure and post-traductional modifications (e.g. phosphorylation, glycosylation level, etc.). Oligo(ethylene glycol) (oEG) films are well known to be efficient at reducing the nonspecific adsorption of biomolecules. However, self-assembled monolayers of thiolated oEG present important limitations related to their lack of stability. To prevent the nonspecific adsorption of the many proteins and biomolecules present in biological fluids, we use a strategy that consists in the modification of surfaces through the grafting of calix[4]arene-tetra-diazonium salts.1 More precisely, we have investigated the grafting on Ge and Au surfaces of monolayers of calix[4]arenes bearing oEGs substituents of different lengths. The anti-fouling properties of these modified surfaces were evaluated by FTIR spectroscopy andconfocal laser scanning fluorescence microscopy upon exposure to bovine serum albumin (BSA) in phosphate (PBS) buffer.