par André, Josette 
Editeur scientifique Rigopoulos, Dimitrios;Elewski, B.;Richert, Bertrand
Référence Onychomycosis diagnosis and effective management, Wiley Blackwell, Oxford, Ed. 1, page (60-65)
Publication Publié, 2018
Editeur scientifique Rigopoulos, Dimitrios;Elewski, B.;Richert, Bertrand
Référence Onychomycosis diagnosis and effective management, Wiley Blackwell, Oxford, Ed. 1, page (60-65)
Publication Publié, 2018
Partie d'ouvrage collectif
| Résumé : | This chapter focuses on the histopathologic diagnosis of onychomycosis. It also focuses on other modalities that include direct examination, specimen culture, molecular methods including polymerase chain reaction (PCR), and matrix-assisted laser desorption/ionization time-offlight mass spectrometry (Maldi Tof MS). The chapter then focuses on the new imaging techniques like optical coherence tomography (OCT) and reflectance confocal microscopy (RCM). Nail plate sampling stained with Periodic acid-Schiff staining (PAS) is a useful complementary technique. PAS reveals the neutral mucopolysaccharides present in the fungal wall. PAS is a useful technique in the diagnosis of onychomycosis, especially when there is strong clinical suspicion in the setting of a negative fungal culture and/or potassium hydroxide (KOH) preparation. The chapter expresses that it is the method with the highest sensitivity in detection of fungal elements in nail specimens, among the routinely available methods. |
