par Busson, Laurent ;Bartiaux, M.;Brahim, Jaime S;Konopnicki, Deborah ;Dauby, Nicolas ;Gerard, Michèle ;De Backer, Paul ;Van Vaerenbergh, K;Mahadeb, Bhavna ;De Foor, Marc;Wautier, M.;Vandenberg, Olivier ;Mols, Pierre ;Levy, Jessica;Hallin, Marie
Référence Journal of virological methods, 266, page (1-6)
Publication Publié, 2019-04
Référence Journal of virological methods, 266, page (1-6)
Publication Publié, 2019-04
Article révisé par les pairs
Résumé : | Aim: To compare the performances of molecular and non-molecular tests to diagnose respiratory viral infections and to evaluate the pros and contras of each technique. Methods: Two hundred ninety-nine respiratory samples were prospectively explored using multiplex molecular techniques (FilmArray Respiratory Panel, Clart Pneumovir), immunological techniques (direct fluorescent assay, lateral flow chromatography) and cell cultures. Findings: Molecular techniques permitted the recovery of up to 50% more respiratory pathogens in comparison to non-molecular methods. FilmArray detected at least 30% more pathogens than Clart Pneumovir which could be explained by the differences in their technical designs. The turnaround time under 2 hours for the FilmArray permitted delivery of results when patients were still in the emergency room. |