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Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors

par Dubois, Arnaud;Levecq, Olivier;Azimani, Hicham;Siret, David;Barut, Anaiss;Suppa, Mariano ;Del Marmol, Véronique ;Malvehy, Josep;Cinotti, Elisa;Rubegni, Pietro;Perrot, Jean Luc Uc J.L.
Référence Journal of biomedical optics, 23, 10, 106007
Publication Publié, 2018-10

Article révisé par les pairs

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Résumé :

An optical technique called line-field confocal optical coherence tomography (LC-OCT) is introduced for high-resolution, noninvasive imaging of human skin in vivo. LC-OCT combines the principles of time-domain optical coherence tomography and confocal microscopy with line illumination and detection using a broadband laser and a line-scan camera. LC-OCT measures the echo-time delay and amplitude of light backscattered from cutaneous microstructures through low-coherence interferometry associated with confocal spatial filtering. Multiple A-scans are acquired simultaneously while dynamically adjusting the focus. The resulting crosssectional B-scan image is produced in real time at 10 frame/s. With an isotropic spatial resolution of ∼1 μm, the LC-OCT images reveal a comprehensive structural mapping of skin at the cellular level down to a depth of ∼500 μm. LC-OCT has been applied to the imaging of various skin lesions, in vivo, including carcinomas and melanomas. LC-OCT images are found to strongly correlate with conventional histopathological images. The use of LC-OCT as an adjunct tool in medical practice could significantly improve clinical diagnostic accuracy while reducing the number of biopsies of benign lesions.