Résumé : In this paper we study the asymptotic and qualitative properties of least energy radial sign-changing solutions of the fractional Brezis–Nirenberg problem ruled by the s-Laplacian, in a ball of Rn, when s∈(0,1) and n>6s. As usual, λ is the (positive) parameter in the linear part in u. We prove that for λ sufficiently small such solutions cannot vanish at the origin, we show that they change sign at most twice and their zeros coincide with the sign-changes. Moreover, when s is close to 1, such solutions change sign exactly once. Finally we prove that least energy nodal solutions which change sign exactly once have the limit profile of a “tower of bubbles” as λ→0+, i.e. the positive and negative parts concentrate at the same point with different concentration speeds.