par Vincenzi, Bruno;Iuliani, Michele;Zoccoli, Alice;Pantano, Francesco;Fioramonti, Marco ;De Lisi, Delia;Frezza, Anna Maria;Rabitti, Carla;Perrone, Giuseppe;Onetti Muda, Andrea;Russo, Antonio;Giordano, Antonio;Santini, Daniele;Dei Tos, Angelo Paolo;Tonini, Giuseppe
Référence Oncotarget, 6, 12, page (10586-10591)
Publication Publié, 2015-04
Référence Oncotarget, 6, 12, page (10586-10591)
Publication Publié, 2015-04
Article révisé par les pairs
Résumé : | Liposarcoma (LPS) is the most common soft tissue sarcoma. It has been demonstrated that mir-155 was the most overexpressed miRNA in well-differentiated LPS(WDLPS)/dedifferentiated LPS (DDLPS). The aim of this study is to evaluate the involvement of Dicer, Drosha and mir-155 in development of LPS and their possible role in stratification of different histological subtypes. Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors. The experimental data were obtained using qRT-PCR comparing Dicer, Drosha and mir-155 expression levels in tumor samples versus normal fat. The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ. Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS. These findings demonstrate for the first time that Dicer is deregulated in LPS and show that mir-155 is differentially expressed in LPS subgroups and it could be a promising tool to improve LPS disease stratification and differential diagnosis. |