Cellobiohydrolase and endoglucanase respond differently to surfactants during the hydrolysis of cellulose.

Hsieh, Chia-Wen; Cannella, David; Jørgensen, Henning; Felby, Claus; Thygesen, Lisbeth LG

doi:doi/10.1186/s13068-015-0242-y

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Cellobiohydrolase and endoglucanase respond differently to surfactants during the hydrolysis of cellulose.

par Hsieh, Chia-Wen ;Cannella, David

;Jørgensen, Henning ;Felby, Claus ;Thygesen, Lisbeth LG
Référence Biotechnology for Biofuels, 8, page (52)
Publication Publié, 2015

Article révisé par les pairs

Résumé :

Non-ionic surfactants such as polyethylene glycol (PEG) can increase the glucose yield obtained from enzymatic saccharification of lignocellulosic substrates. Various explanations behind this effect include the ability of PEG to increase the stability of the cellulases, decrease non-productive cellulase adsorption to the substrate, and increase the desorption of enzymes from the substrate. Here, using lignin-free model substrates, we propose that PEG also alters the solvent properties, for example, water, leading the cellulases to increase hydrolysis yields.

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