par Song, Yongli;Xiao-ning, He;Song, Hua;Lan, Jie 
;Liu, Yonggang;Cheng, Pang;Zhang, Hailin;Li, Jixia;He, Xiaoying ;Liu, Jun;Zhang, Yong
Référence Zygote, 21, 3, page (265-269)
Publication Publié, 2013-08
;Liu, Yonggang;Cheng, Pang;Zhang, Hailin;Li, Jixia;He, Xiaoying ;Liu, Jun;Zhang, Yong Référence Zygote, 21, 3, page (265-269)
Publication Publié, 2013-08
Article révisé par les pairs
| Résumé : | The purpose of this study was to prepare intracellular pathogen resistance 1 (Ipr1) transgenic donor cells for somatic cell nuclear transfer (SCNT). Based on our current understanding of Ipr1, a macrophage special expression vector pSP-EGFP-Ipr1was constructed. Bovine fetal fibroblasts were transfected with pSP-EGFP-Ipr1. The green fluorescent protein (GFP)-expressing cells were selected and transferred into enucleated bovine oocytes. Then, the rates of oocyte cleavage and blastocyst formation of transgenic cells and non-transgenic cells were observed, respectively. The results showed that reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. This study may provide cloned embryos for the production of anti-tuberculosis transgenic animals. |
