par Wacquier, Benjamin 
;Romero Campos, Hugo H.E.;González-Vélez, Virginia;Combettes, Laurent;Dupont, Geneviève
Référence The FEBS journal, 284, 23, page (4128-4142)
Publication Publié, 2017-12
;Romero Campos, Hugo H.E.;González-Vélez, Virginia;Combettes, Laurent;Dupont, Geneviève Référence The FEBS journal, 284, 23, page (4128-4142)
Publication Publié, 2017-12
Article révisé par les pairs
| Résumé : | Mitochondria play a significant role in shaping cytosolic Ca2+ signals. Thus, transfer of Ca2+ across the mitochondrial membrane is much studied, not only in intact cells but also in artificial systems such as mitochondrial suspensions or permeabilised cells. Observed rates of Ca2+ changes vary by at least one order of magnitude. In this work, we investigate the relationship between the Ca2+ dynamics observed in various experimental conditions using a computational model calibrated on experimental data. Results confirm that mitochondrial Ca2+ exchange fluxes through the mitochondrial Ca2+ uniporter (MCU) and the Na+/Ca2+ exchanger obey the same basic kinetics in cells and in suspensions, and emphasise the important role played by the high Ca2+ levels reached in mitochondria-associated endoplasmic reticulum membranes in intact cells. Tissue specificity can be ascribed to the different modes of regulation of the MCU by Ca2+, probably related to the specific levels of expression of the Ca2+ sensing regulator subunit of this channel. The model emphasises the importance of mitochondrial density and buffering in controlling the rate of Ca2+ exchanges with mitochondria, as verified experimentally. Finally, we show that heterogeneity between individual mitochondria can explain the large range of amplitudes and rates of rise in mitochondrial Ca2+ concentration that have been observed experimentally. |
