• Citer

Deoxyribonucleic acid of the crab Cancer pagurus. III. Intracellular localization of poly d(AT) of Cancer pagurus by hybridization in situ

par Chevaillier, Ph;de Recondo, Anne Marie;Geuskens, Maurice
Référence Experimental cell research, 86, 2, page (383-391)
Publication Publié, 1974

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

The satellite DNA poly [d(AT) · d(TA)] of the crab Cancer pagurus has been localized in situ by DNA-DNA hybridization in the nuclei of various spermatogenetic, midgut gland, intestinal and tegument cells. The specificity of hybridization was checked by various tests before, during and after hybridization. The nuclear sites revealed by this method were compared with those shown by quinacrine mustard or Giemsa staining. The AT-rich satellite DNA appears to be highly dispersed and does not seem to have any preferential localization inside the crab interphasic nucleus. This situation was compared with that presented by mouse nuclei using similar methods. © 1974.