par Maenhaut, Geneviève ;Caillet Fauquet, Perrine
Référence Journal of Molecular Biology, 177, 1, page (181-187)
Publication Publié, 1984-07
Référence Journal of Molecular Biology, 177, 1, page (181-187)
Publication Publié, 1984-07
Article révisé par les pairs
Résumé : | Mutagenesis of phage λ towards clear-plaque phenotype (c+ → c) results in two classes of mutants that can be distinguished genetically and morphologically. Indirect mutagenesis i.e. mutagenesis of unirradiated phage λc+ stimulated by the ultraviolet irradiation of the Escherichia coli host, results in mixed bursts ( c c+) of turbid wild-type and clear-plaque mutant phages. Pure bursts of λc mutants are induced by irradiation of the phage genome. Irradiation of both phages and host bacteria stimulates the production of the two classes of mutant clones. We show that three different mutant alleles of the E. coli umuC gene only prevent the appearance of pure bursts of clear-plaque mutants, while mixed bursts are produced at least as frequently in umuC mutants as in the umuC+ parent. © 1984. |