par Vandermeers, André 
;Vandermeers-Piret, Marie-Claire ;Christophe, Jean
Référence Clinica chimica acta, 37, C, page (471-476)
Publication Publié, 1972
;Vandermeers-Piret, Marie-Claire ;Christophe, Jean Référence Clinica chimica acta, 37, C, page (471-476)
Publication Publié, 1972
Article révisé par les pairs
| Résumé : | 1. 1. An automated method was developed for the estimation of enzymatic activities based on the slope measurement of linear reaction curves. The procedure is applicable to most spectrophotometric assays, even when enzymatic reaction velocity is measured by means of a subsequent chemical reaction. 2. 2. The method was developed in the particular case of soluble starch hydrolysis by rat pancreatic amylase, estimated by the reduction of 3,5-dinitrosalicylic acid. Good kinetic graphs were obtained at a rate of 12 assays per hour for 1.8 min recording time. © 1972. |
