Résumé : A spin‐echo based 1H homonuclear pulse sequence, enabling the selective editing of homonuclear first‐order J‐multiplet types, is presented and analyzed. Its effectiveness in quantification applications is assessed. Its potential usefulness in the quantitative distinction between 2‐deuterated and natural D‐,glucose in biological samples is briefly discussed, with emphasis on studies of hexose metabolism conducted in vitro. Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company