par Craenhals, Edgard 
;Leonis, José
Référence Bulletin des Sociétés chimiques belges, 64, 1-2, page (58-69)
Publication Publié, 1955
;Leonis, José Référence Bulletin des Sociétés chimiques belges, 64, 1-2, page (58-69)
Publication Publié, 1955
Article révisé par les pairs
| Résumé : | The behaviour of the protein enzyme lysozyme towards countercurrent distribution was investigated. Several solvent systems were discarded, owing to poor solubility of the enzyme, denaturation, or/and unfavourable distribution coefficient. A mixture of 2.butanol, water, and p. toluenesulfonic acid was however found efficient. It is established that none of the components of the last mentioned system has inhibiting action upon enzymic activity of the protein. It is further shown that the distribution coefficient of lysozyme is, within limits, a linear function of the acid molarity. This allowed a fractionation of the protein into three components, one of which amounts to nearly 80 %. Copyright © 1955 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim |
