par Bienz, Mariann;Kubli, Eric;Kohli, Jürg;De Henau, Suzanne;Grosjean, Henri 
Référence Nucleic acids research, 8, 22, page (5169-5178)
Publication Publié, 1980-11
Référence Nucleic acids research, 8, 22, page (5169-5178)
Publication Publié, 1980-11
Article révisé par les pairs
| Résumé : | Amber, ochre, and opal nonsense suppressor tRNAs isolated from yeast were injected into Xenopus laevis oocytes together with purified mRNAs (globin mRNA from rabbit, tobacco mosaic virus-RNA). Yeast opal suppressor tRNA is able to read the UGA stop codon of the rabbit β-globin mRNA, thus producing a readthrough protein. A large readthrough product is also obtained upon coinjection of yeast amber or ochre suppressor tRNA with TMV-RNA. The amount of readthrough product is dependent on the amount of injected suppressor tRNA. The suppression of the terminator codon of TMV-RNA is not susceptible to Mg++ concentration or polyamine addition. Therefore, the Xenopus laevis oocyte provides a simple, sensitive, and well buffered in vivo screening system for all three types of eukaryotic nonsense suppressor tRNAs. © 1980 IRL Press Limited. |
