Article révisé par les pairs
Résumé : Intravenous immunization of rabbits with Micrococcus lysodeikticus results in the production of antibodies of electrophoretically restricted heterogeneity. In some cases up to 30 mg antibody per ml serum are obtained. Major antibody fractions studied here have specificity directed against Micrococcus‐ specific carbohydrate antigen. Antibody absorbed on cell walls can be specifically eluted with N‐acetylglucosamine solution. Both glucose and N‐acetylglucosamine are shown to inhibit antibody‐cell wall carbohydrate precipitin reaction. The isoelectric focusing pattern of specific antibody obtained from an immune precipitate exhibit a limited number of bands. Antibody light and heavy chains yield single bands on acrylamide disc electrophoresis. Copyright © 1975, Wiley Blackwell. All rights reserved