par Gaspard, Pierre 
Référence Physical review. E, Statistical, nonlinear, and soft matter physics, 93, 4, 042420l
Publication Publié, 2016-04
Référence Physical review. E, Statistical, nonlinear, and soft matter physics, 93, 4, 042420l
Publication Publié, 2016-04
Article révisé par les pairs
| Résumé : | Kinetic theory and thermodynamics are applied to DNA polymerases with exonuclease activity, taking into account the dependence of the rates on the previously incorporated nucleotide. The replication fidelity is shown to increase significantly thanks to this dependence at the basis of the mechanism of exonuclease proofreading. In particular, this dependence can provide up to a 100-fold lowering of the error probability under physiological conditions. Theory is compared with numerical simulations for the DNA polymerases of T7 viruses and human mitochondria. |
