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Colony-inhibiting activity of polymorphonuclear cells in chronic myeloid leukemia

par Stryckmans, Pierre ;Peyer, Andreas;Collard Ronge, E.
Référence Blood cells, 3, 3, page (493-504)
Publication Publié, 1977

Article révisé par les pairs

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Résumé :

Eight studies were performed on the blood of five patients with Ph1 positive chromosome chronic myeloid leukemia (CML) in remission induced by either hydroxyurea, 6-thioguanine, or busulfan. The culture system was a modification of the classical Pike and Robinson assay. Cultures with stimulating mononuclear blood cells (buoyant density <1.077) in the feeder-layer, and target-marrow from the same normal donor in the overlayer were used. A third layer composed of polymorphonuclear cells (PMN) (dense, density >1.077 and <1.120) from CML patients or normal controls was inserted between the mononuclear feeder-layer and the marrow-containing overlayer. Addition of increasing numbers of PMN from either CML patients or normal controls caused a similar progressive depression in the number of colonies and macro-clusters. Identical results were obtained in three non-stimulated cultures and one PMN partially depleted bone-marrow. The present studies thus indicate that, in remission of CML, there are PMN with a normal marrow-inhibiting activity. Therefore, a regulation defect of granulopoiesis in CML probably cannot be attributed to a defect of the PMN in the physiological negative feed-back they exert.