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Detection of mecA-and mecC-positive methicillin-resistant staphylococcus aureus (MRSA) isolates by the new Xpert MRSA Gen 3 PCR assay

par Becker, Karsten;Schaumburg, Frieder;Peters, Georg;Ballhausen, Britta;Denis, Olivier ;Roisin, Sandrine ;Mellmann, Alexander;Idelevich, Evgeny E.A.;Knaack, Dennis;Van Alen, Sarah;Kriegeskorte, André;Köck, Robin
Référence Journal of clinical microbiology, 54, 1, page (180-184)
Publication Publié, 2016-01

Article révisé par les pairs

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Résumé :

An advanced methicillin-resistant Staphylococcus aureus (MRSA) detection PCR approach targeting SCCmec-orfX along with mecA and mecC was evaluated for S. aureus and coagulase-negative staphylococci. The possession of mecA and/or mecC was correctly confirmed in all cases. All methicillin-susceptible S. aureus strains (n=98; including staphylococcal cassette chromosome mec element [SCCmec] remnants) and 98.1% of the MRSA strains (n=160, including 10 mecC-positive MRSA) were accurately categorized.