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Identification of a second Mycobacterium tuberculosis gene cluster encoding proteins of an ABC phosphate transporter.

par Braibant, Martine ;Lefèvre, Philippe ;De Wit, Lucas;Ooms, Josette;Peirs, Priska ;Huygen, Kris ;Wattiez, Ruddy ;Content, Jean
Référence FEBS letters, 394, 2, page (206-212)
Publication Publié, 1996-09

Article révisé par les pairs

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Résumé :

Following the identification of a M. tuberculosis phosphate transporter belonging to the superfamily of ABC transporters, we report on the cloning and sequencing of two additional genes, called pstS-3 and pstC-2, encoding proteins homologous to PstS and PstC of Escherichia coli, respectively. Together with the previously isolated M. tuberculosis gene similar to the E. coli pstA, these are included in a cluster encoding a second putative phosphate transport system. We demonstrate that pstS-3 encodes the previously described Ag 88, a 40 kDa M. bovis BCG culture filtrate antigen (immunodominant in H-2b haplotype type mice). Finally, a signature motif identifying integral transmembrane proteins of prokaryotic phosphate binding-dependent permeases is proposed.