par Huez, Georges 
;Burny, Arsène ;Marbaix, Gérard ;Schram, Eric
Référence European journal of biochemistry / FEBS, 1, 2, page (179-181)
Publication Publié, 1967-04
;Burny, Arsène ;Marbaix, Gérard ;Schram, Eric Référence European journal of biochemistry / FEBS, 1, 2, page (179-181)
Publication Publié, 1967-04
Article révisé par les pairs
| Résumé : | Two methods are described for the rapid isolation of 0.6 mg to 1 mg of 9S RNA from rabbit reticulocytes. This RNA has properties expected for messenger RNA. In the first procedure, reticulocyte polyribosomes are dissolved in 0.5% (w/v) sodium dodecylsulfate and messenger RNA is separated by sucrose gradient centrifugation in a zonal rotor (B IV‐Spinco). In the second method, messenger RNA is detached from polyribosomes by EDTA (3x10−2M) treatment and separated from ribosomal sub‐particles by sucrose gradient centrifugation using the same rotor. In both cases, messenger RNA is then concentrated by adsorption on a non‐ionic cellulose column. Further purification may be obtained by sucrose gradient centrifugations using the rotor S. W. 25.2 (Spinco). Copyright © 1967, Wiley Blackwell. All rights reserved |
