• Citer

Development and Characterization of Microsatellite Markers in the African Deciduous Tree Terminalia superba (Combretaceae)

par Demenou, Boris ;Migliore, Jérémy ;Tosso, Félicien;Kaymak, Esra ;Hardy, Olivier J.
Référence Applications in plant sciences, 3, 1500070
Publication Publié, 2015-12-09

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

• Premise of the study: Microsatellites were designed and characterized in the African timber forest tree Terminalia superba(Combretaceae). Due to their high variability, these markers are suitable to investigate gene fl ow patterns and the structure ofgenetic diversity.• Methods and Results: From a genomic library obtained by next-generation sequencing, seven monomorphic and 14 polymorphicmicrosatellite loci were developed. The polymorphic microsatellites displayed two to 27 alleles (mean 11.4; expectedheterozygosity range 0.283–0.940, mean 0.736) in one population from southeastern Cameroon. Genotypes were typical ofan outbreeding diploid species, although null alleles explain a signifi cant heterozygote defi cit in three loci. Cross-amplifi cationin three congeneric species ( T. ivorensis , T. avicennioides , and T. mantaly ) failed, suggesting that T. superba is rather divergent.• Conclusions: This set of newly developed microsatellite markers will be useful for assessing the genetic diversity, populationstructure, and demographic history of T. superba in tropical African forests.