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Immobilised cyclin-dependent kinase 4 fusion proteins and uses thereof

par Raspé, Eric ;Roger, Pierre P. ;Coulonval, Katia ;Paternot, Sabine ;Bisteau, Xavier
Publication Publié, 2015-10-22

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Résumé :

The present invention concerns an in vitro assay for determining the activation status of endogenous CDK4 in eukaryotic cells, said assay comprising the steps of: providing eukaryotic cells maintained in a quiescent state, said eukaryotic cells comprising a cyclin D/CDK4 fusion protein, wherein the CDK4 part of the fusion protein is present in a hypophosphorylated form; inducing proliferation of said eukaryotic cells; isolating the cyclin D/CDK4 fusion protein from said eukaryotic cells; and measuring the activation status of said isolated cyclin D/CDK4 fusion protein, thereby determining the activation stats of endogenous CDK4 in said eukaryotic cells.