Article révisé par les pairs
Résumé : [en] This study investigates the mesophilic biohydrogen production from glucose using astrictly anaerobic strain, Clostridium butyricum CWBI1009, immobilized in a trickling bedsequenced batch reactor (TBSBR) packed with a Lantec HD Q-PACtextregistered packing material(132 ft2/ft3 specific surface). The reactor was operated for 62 days. The main parametersmeasured here were hydrogen composition, hydrogen production rate and soluble metabolicproducts. pH, temperature, recirculation flow rate and inlet glucose concentration at10 g/L were the controlled parameters. The maximum specific hydrogen production rateand the hydrogen yield found from this study were 146 mmol H2/L.d and 1.67 mol H2/molglucose. The maximum hydrogen composition was 83%. Following a thermal treatment,the culture was active without adding fresh inoculum in the subsequent feeding and boththe hydrogen yield and the hydrogen production rate were improved. For all sequences, thesoluble metabolites were dominated by the presence of butyric and acetic acids comparedto other volatile fatty acids. The results from the standard biohydrogen production (BHP)test which was conducted using samples from TBSBR as inoculum confirmed that theculture generated more biogas and hydrogen compared to the pure strain of C. butyricumCWBI1009. The effect of biofilm activity was studied by completely removing (100%) themixed liquid and by adding fresh medium with glucose. For three subsequent sequences,similar results were recorded as in the previous sequences with 40% removal of spentmedium. The TBSBR biofilm density varied from top to bottom in the packing bed and thehighest biofilm density was found at the bottom plates. Moreover, no clogging was evidencedin this packing material, which is characterized by a relatively high specific surfacearea. Following a PCA test, contaminants of the Bacillus genus were isolated and a standardBHP test was conducted, resulting in no hydrogen production.